This work was done for optimizing the route to produce eco-friendly bacterial cellulose from bacteria in the rotten guava. and protein waste as cheap carbon/ nitrogen source in production medium. In this respect Komagataeibacter intermedius MO was isolated and identified. The protein-based waste (cheese whey) was evaluated as effective carbon/ nitrogen source in growth medium. https://www.flippsinfurnitureshops.shop/product-category/tissue-paper/